The NGS Targeted Capture Process involves using blockers for adapters to prevent the binding between adapter sequences in the library. Additionally, Cot-1 DNA is employed to block high-copy regions/low-complexity regions of library insert fragments, thereby preventing non-specific capturing of such regions caused by probe cross-hybridization.
By significantly reducing these two types of non-specific bindings, the on-target rate and data utilization can be improved. Dynegene Technology provides blocker reagents for targeted capture on both Illumina and MGI platforms. Moreover, they offer separate human Cot-1 DNA to support multiplexed hybridization, thus ensuring efficient capture for users.
Key Features
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Excellent Blocking Performance
By blocking multiple adapter sequences and regions with a high genome copy rate or low genome sequence complexity, the non-specific binding between different library molecules is effectively reduced, and the capture efficiency is improved.
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Versatile Applications
We offer blockers tailored for various sequencing platforms, including Illumina and MGI, and compatible with different technical processes, such as single hybridization and multiple hybridization.
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Lower Cost
Universal blockers can be used to block multiple-hybridization libraries, and an alternative for single-hybridization can be provided, reducing the cost of each sample.
Blocking schematic
NGSHybridization Capture DNA Probe Hybridization Capture RNA Probe QuarXeq Human All Exon Probes 3.0 HRD panel Library Preparation DNA Library Preparation Kit Fragmentation Reagent RNA Library Preparation Kit mRNA Capture Kit rRNA Depletion Kit QuarPro Superfast T4 DNA Ligase Hybridization Capture QuarHyb DNA Plus 2 Reagent Kit QuarHyb DNA Reagent Kit Plus Hybridization and Cleaning Solution System QuarHyb Super Reagent Kit Pro Dynegene Adapter Family Dynegene Blocker Family Multiplex PCR QuarMultiple BRCA Amplicon QuarMultiple PCR Capture Kit 2.0 PathoSeq 450 Pathogen Library Corollary Reagent Streptavidin magnetic beads Equipment and Software The iQuars50 NGS Prep System
Primers and Probes
RNA SynthesissgRNA miRNA siRNA
Gene Synthesis
Oligo Pools
CRISPR sgRNA Library
Antibody Library
Variant Library
Tel: 400-017-9077
Address: Floor 2, Building 5, No. 248 Guanghua Road, Minhang District, Shanghai
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