• Good performance in capture

Figure 1. The capturing performance of QuarXeq HRD Probes

Note: Input 100ng of standard (NA12878) was used for ultrasonic DNA library preparation (QuarPrep DNA Library Kit, Dynegene, NL1001). 750 ng of prepared library and QuarXeq HRD Probes were used for routine hybridization capture (QuarHyb One Reagent Kit, Dynegene, NC1003),The test was repeated three times, and sequencing was performed on illumina platform, The data volumn used for analysis was 6 G.

 

• The ratio of HRR to HRD sequencing depth under differential capture is consistent with expectation

 

Figure 2. The sequencing depth under differential capture using QuarXeq HRD Probes

Note: Input 100 ng of standard (NA12878) was used for ultrasonic DNA library preparation (QuarPrep DNA Library Kit, Dynegene, NL1001). 750 ng of prepared library and QuarXeq HRD Probes were used for routine hybridization capture (QuarHyb One Reagent Kit, Dynegene, NC1003),The test was repeated three times, and sequencing was performed on illumina platform. The results showed that: The ratio of HRRto HRD sequencing_depth was 5:1,which was consistent with expectation.

Figure 3. The comparison of HRD BRCA2 sequencing depth with Chr 13 under differential capture using QuarXeq HRD Probes

The sequencing depth of HRD BRCA2 and Chr 13 were analyzed ,and the result showed that the ratio of the two vales was 5:1, which was also consistent with expectation.

 

• Uniform coverage of SNP sites

Figure 4. The coverage of SNP Marker chromosome

Note: in this figure, the gray areas are highly repetitive areas on the reference genome, the scattered blue dots are SNP Marker MAF repeat elements.QuarXeq HRD Probes中SNPsThe SNP sites are uniformly distributed in the QuarXeq HRD Probes, to cover as many of heterozygous sites as possible, and ensure that LOH, LST and TAI that meet the requirements can be detected.