• Compatible with different modules of RNA enrichment. High yield can be obtained even at an input of 1ng

RNA standards (Agilent, Item No.: 740000-41) are sampled for the library preparation of general transcriptomics at different inputs. mRNA enrichment (NL3011, Dynegene) and rRNA removal (NL3006, Dynegene) are selected as front-end enrichment strategies respectively, which then can be used for RNA library preparation (NL3008, Dynegene). The results showed that high yield can be obtained after RNA library preparation at different inputs and be combined with different modules of RNA enrichment. High yield can also be obtained even at the limit input of 1ng.

 

• High library yield and the peak profile are consistent with expectations

 

RNA standards (Agilent, Item No.: 740000-41) are selected as samples at an input of 100ng. A complete set of RNA enrichment library preparation kits are used for the library preparation of general transcriptomics respectively according to the respective instructions of Dynegene and supplier (A). It is estimated that the length of inserted sequence is 250-350bp with 13 PCR cycles and the sequencing is performed on an Illumina platform. The results showed that Dynegene delivers higher library yield than supplier A . The peak profile is consistent with expectation while that of supplier (A) tends to be small.

 

• Compatible with different sequencing platforms

RNA standards (Agilent, Item No.: 740000-41) are sampled for the library preparation of general transcriptomics at different inputs. rRNA removal (NL3006, Dynegene) is selected as front-end enrichment strategy, which then can be used for RNA library preparation (NL3008, Dynegene). Adapters for Illumina platform (NA2002, Dynegene) and adapters for BGI (NL3008, Dynegene) platform are used as adapters and primers respectively. The results showed that Dynegene RNA library preparation kit, combined with adapters for Illumina and BGI platform, could deliver high yield library.

 

• Excellent data quality

RNA standards (Agilent, Item No.: 740000-41) are selected as samples at an input of 100ng. RNA library preparation is performed according to the respective instructions of Dynegene and supplier (A). And the sequencing is performed on an Illumina platform. The data showed that detected genes of Dynegene are slightly higher than that of supplier (A) while the adapter rate of Dynegene is much lower than that of supplier (A).