DNA mutagenesis, especially, site-direct mutagenesis of DNA sequence is widely used to protein and nucleic acid engineer. Degenerate codon (NNK) and trinucleotide randomize DNA sequence coded for specific amino acid are two common methods to study specific AA or domain function of protein. However, the degenerate codon can’t precisely synthesize specific codons, and trinucleotide has restricted codon usage. Moreover, both of these two methods always amplify library size that it will be hard to design screening method to find candidate in practice when we want to study the function of combined multiple AA sites within protein.

Array-based oligo pools not only precisely synthesize DNA fragment with any codon but also remove unwanted codon. Thus it will largely decrease the library size and make candidate screening more easily from the library. Dynegene technology now provide 230 bps DNA oligo pools synthesis with low error rate ( ~ 0.1% bps) and the cost is about 10% of conventional DNA oligo synthesis. In addition, Dynegene technology provide service of constructing oligo pools into phage-displayed, yeast displayed, as well as mammalian displayed plasmid. Service of biopanning through phage-displayed libraries is also provided by us. All of this is to help pharmaceutics companies to rapidly evolve to provide more solution in combating diseases. Please contact us for more detail.