The NGS Targeted Capture Process involves using blockers for adapters to prevent the binding between adapter sequences in the library. Additionally, Cot-1 DNA is employed to block high-copy regions/low-complexity regions of library insert fragments, thereby preventing non-specific capturing of such regions caused by probe cross-hybridization.
By significantly reducing these two types of non-specific bindings, the on-target rate and data utilization can be improved. Dynegene Technology provides blocker reagents for targeted capture on both Illumina and MGI platforms. Moreover, they offer separate human Cot-1 DNA to support multiplexed hybridization, thus ensuring efficient capture for users.
Key Features
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Excellent Blocking Performance
By blocking multiple adapter sequences and regions with a high genome copy rate or low genome sequence complexity, the non-specific binding between different library molecules is effectively reduced, and the capture efficiency is improved.
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Versatile Applications
We offer blockers tailored for various sequencing platforms, including Illumina and MGI, and compatible with different technical processes, such as single hybridization and multiple hybridization.
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Lower Cost
Universal blockers can be used to block multiple-hybridization libraries, and an alternative for single-hybridization can be provided, reducing the cost of each sample.
Blocking schematic