In the NGS targeted capture process, the use of blockers for adapters can prevent the binding between adapter sequences in the library, while Cot-1 DNA can block high-copy regions/low-complexity regions of library insert fragments, preventing non-specific capturing of such regions caused by probe cross-hybridization. By significantly reducing these two types of non-specific bindings, the on-target rate and data utilization can be improved. Dynegene Technologies provides the blocker reagents for targeted capture on both illumina and MGI platform, and also offers separate human Cot-1 DNA to support multiplexed hybridization, ensuring efficient capture for users.
Key Features
-
Excellent blocking performance
By blocking multiple adapter sequences and regions with a high genome copy rate or low genome sequence complexity, the non-specific binding between different library molecules is effectively reduced, and the capture efficiency is improved
-
Flexible applications
We provide blockers adapted to different sequencing platforms such as Illumina and MGI, and different technical processes such as single hybridization and multiple hybridization
-
Lower cost
Universal blocker can be used to block multiple-hybridization libraries, and an alternative for single-hybridization can be provided which reduce the cost of each sample
Blocking schematic