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Gene Synthesis

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Overview

Dynegene Technologies, based on years of dedicated research and development, has launched the new generation gene synthesis platform DGS 2.0. This platform is different from traditional gene synthesis as it can balance the demands of both throughput and yield. With its independently developed ultra-redundant HiFi assembly technology, combined with a capability to synthesize up to 400nt long sequences and rich experience in gene synthesis, it can provide customers with gene synthesis services ranging from 0.3-10KB, with various delivery options.

 

Features of Dynegene Gene Synthesis platform DGS 2.0

● Ultra-long synthesis technology

Can solve the synthesis of antibody gene fragments and difficult sequences.

400nt

● High cost-effective synthesis volume

Different from the high cost waste of nmol-level column synthesis and the complex amplification and depooling required by fmol-level chip-based synthesis, pmol-level synthesis is more suitable.

 

pmol级

● High-throughput cluster primer synthesis

Can achieve in situ joining of hundreds of genes and downstream automated rapid synthesis.

6-24k

● HiFi assembly process

Independent research and development of ultra-redundant assembly and multi-point error correction process, lead to the fragment accuracy rate up to 98%.

98%

 

 

Highlights

 

 

Service Content

 

Delivery form

Length of Fragments

Service Description

Delivery Standard

Fragment

<0.5k

Synthesis of double-stranded DNA fragments

Default delivery of 30-100ng double-stranded DNA fragments; COA file.

0.5k-1.5k

1.5-2k

Cloning vector

<0.5k

Delivery of target genes cloned onto vector plasmids

Default delivery of 4-5μg lyophilized plasmid DNA (2-4μg for low-copy plasmid DNA); sequencing data, sequence comparison files, plasmid structure files, COA file.

0.5k-1.5k

1.5-3k

3-5k

Validated fragment

<0.5k

Obtaining target gene fragments from validated correct plasmids through digestion or PCR methods

Default delivery of 200ng target gene fragments; sequence comparison files, COA file.

0.5k-1.5k

1.5-3k

 

*For sequences exceeding 5KB, please inquire for details.

 

 

Other optional services

 

Free codon optimization: Dynegene Technologies can optimize codons based on different host codon preferences to optimize protein expression.

Large-scale preparation of plasmid DNA: In addition to the regular gene synthesis delivery volume, Dynegene Technologies can provide different scales of plasmid DNA preparation services.

Subcloning service: Dynegene Technologies are able to clone your sequences into the selected vectors.

Dynegene Next-Gen Synthesis: Powering Biotech Revolution With Nucleic Acids

Contact Us

Tel: 400-017-9077

Address: Floor 2, Building 5, No. 248 Guanghua Road, Minhang District, Shanghai

Email: zhengyuqing@dynegene.com

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