The QuarCam Plus Seamless Cloning Kit (Ready Mix) is based on the Gibson Assembly principle. By pre-mixing optimized recombination-enhancing factors into the recombination reagents, it enables highly efficient seamless assembly and cloning of 1 to 12 insert fragments (ranging from 50 bp to 12 kb). It also enhances the tolerance of the recombination system to impurities, allowing linearized vectors and insert fragments to be used directly in the recombination reaction without purification, greatly simplifying the experimental procedure.
Seamless Cloning Workflow
Features
-
Multi-Fragment Compatibility
Compatible with the insertion and ligation of 1 to 12 target fragments (50 bp to 12 kb), resulting in a high number of clones and a high positive rate.
-
Simplified Operation
One-tube reaction; linearized vectors and insert fragments can be used directly without purification.
Data
High Cloning Positive Rate with Multi-Fragment Homologous Recombination Compatibility
The QuarCam Plus Seamless Cloning Kit (Ready Mix) (Cat # NG1005) contains pre-mixed optimized recombination-enhancing factors. Multi-fragment assembly experiments were conducted using QuarCam Plus Seamless Cloning Kit (Ready Mix) (Cat # NG1005), a conventional Gibson reagent, and a competitor's reagent. After transformation and plating, colonies were picked for colony PCR. Agarose gel electrophoresis confirmed correct fragment sizes as colony PCR positive. Colony PCR-positive clones were subjected to Sanger sequencing, and clones with perfectly correct recombinant region sequences were scored as positive.
Results show that QuarCam Plus Seamless Cloning Kit (Ready Mix) (Cat # NG1005), with the added enhancing factors, significantly improves the positive clone rate, outperforming the competitor.
|
Correct Assembly Rates for Various Systems (Colony PCR Detection)
|
| Sample |
Gibson+Enhance (10 kb divided into 10 fragments, 1 kb/fragment) |
| System |
Correct Clone Rate |
Empty Vector Rate % (Blue colonies/Total colonies) |
| Conventional GibsonGibson |
60%(6/10) |
64.5%(82/127) |
| Competitor N |
80%(8/10) |
77.9%(92/118) |
| NG1005 |
90%(9/10) |
29.6%(40/135) |
|
Correct Assembly Rates for Various Systems (Fluorescence-Based Detection))
|
| Sample |
Gibson+Enhance (1.5 kb divided into 10 fragments, 170 bp/fragment) |
| System |
Fluorescence-Positive Colonies/Total Colonies |
Positive Rate |
| Conventional GibsonGibson |
322/354 |
90.9%(322/354) |
| Competitor N |
168/189 |
88.9%(168/189) |
| NG1005 |
208/217 |
95.8%(208/217) |
Ordering Information
|
Product Name
|
Cat. No.
|
Format
|
|
QuarCam Plus Seamless Cloning Kit (Ready Mix)
|
NG1005A / NG1005B
|
10rxn / 50rxn
|
NGSHybridization Capture DNA Probe QuarStar Human All Exon Probes 4.0 (Tumor) QuarStar Human All Exon Probes 4.0 (Standard) QuarStar Liquid Pan-Cancer Panel 3.0 QuarStar Pan-Cancer Lite Panel 3.0 QuarStar Pan-Cancer Fusion Panel 1.0 QuarStar Pan Cancer Panel 1.0 Hybridization Capture RNA Probe QuarXeq Human All Exon Probes 3.0 HRD panel Library Preparation DNA Library Preparation Kit Fragmentation Reagent mRNA Capture Kit rRNA Depletion Kit QuarPro Superfast T4 DNA Ligase Hybridization Capture QuarHyb Super DNA Reagent Kit QuarHyb DNA Plus 2 Reagent Kit QuarHyb DNA Reagent Kit Plus QuarHyb One Reagent Kit QuarHyb Super Reagent Kit Pro Dynegene Adapter Family Dynegene Blocker Family Multiplex PCR QuarMultiple BRCA Amplicon QuarMultiple PCR Capture Kit 2.0 PathoSeq 450 Pathogen Library Corollary Reagent Streptavidin magnetic beads Equipment and Software The iQuars50 NGS Prep System
Primers and Probes
RNA SynthesissgRNA miRNA siRNA
Gene Synthesis
Oligo Pools
CRISPR sgRNA Library
Antibody Library
Variant Library
Tel: 400-017-9077
Address: Floor 2, Building 5, No. 248 Guanghua Road, Minhang District, Shanghai
Email: 
