Oligo pools are high-diversity collections of oligonucleotides, which have various applications, such as the CRISPR sgRNA library, variant library, antibody library, massively parallel reporter assays (MPRA), targeted sequencing, fluorescence in situ hybridization (FISH), high-throughput gene synthesis, DNA storage, etc.
Dynegene Oligo pools are synthesized by the high-throughput DNA synthesis platform developed by Dynegene based on DYHOW technology. The platform is capable of synthesizing up to 230 nt and 1.5 million oligos on a single chip with high accuracy and uniformity.
Highlights
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High throughput
1.5 million diverse sequences, each up to 230 nucleotides in length, can be synthesized on a single chip.
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High Quality
Over 99% of the coverage and high uniformity, less than 1/1000 error rate, 0.1-1 fmol per oligo, and rigorous NGS QC processes are in place to guarantee the delivery's quality.
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No re-used Chip
The chips used in the synthesis process are never re-used, ensuring batch-to-batch stability and effectively preventing cross-contamination.
Workflow
Applications
● CRISPR sgRNA Library
sgRNA library construction - consists of thousands of plasmids containing multiple sgRNAs for each target gene. These libraries leverage the efficiency and specificity of the CRISPR gene editing technology to either knock out gene expression or transcriptionally activate genes in the genome. It is particularly valuable for conducting high-throughput screening of important molecular targets.
● Variant Library
Variant libraries are instrumental in synthesizing thousands of gene variants simultaneously, allowing for the identification of key structural and functional sites. These libraries play a prominent role in diverse fields, such as protein engineering, metabolic engineering, and antibody optimization.
● Antibody Library
Synthetic antibody libraries are constructed from scratch using designed synthetic DNA, allowing for the creation of specific antibodies targeted against a wide range of protein antigens. Antibody libraries streamline the creation of specific antibodies derived from natural immunization, serving as strong tools to accelerate the antibody discovery process. This not only simplifies the process but also enhances the efficiency and precision, catalyzing breakthroughs in antibody research.
● Massively Parallel Reporter Assays (MPRA)
The massively parallel reporter assay (MPRA) facilitates the simultaneous functional validation of thousands of regulatory elements through the utilization of high-throughput sequencing and barcode technology. This approach aids researchers in unraveling the functional implications of genetic variation and identifying genetic variants linked to human diseases and traits.
● Targeted Sequencing
Target regions can be efficiently enriched for sequencing by synthesizing specific probes on a high-throughput synthesis platform, resulting in reduced sequencing costs.
● Fluorescence in situ hybridization (FISH)
Oligo pools can be utilized to expedite the development of FISH probes and aid in spatial transcriptome research. For instance, MerFish can identify and analyze gene expression profiles that are spatially localized in individual cells.
● High-throughput Gene Synthesis
The field of synthetic biology is experiencing rapid growth, which has led to an enormous demand for synthetic genes that traditional gene synthesis capabilities cannot keep up with. Fortunately, high throughput oligo synthesis provides the capacity to synthesize massive amounts of genes simultaneously.
● DNA Storage
DNA sequences emerge as a promising medium for data storage, boasting unparalleled attributes, including remarkable density, stability, and durability. Positioned as an alternative to address the challenges of data storage insufficiency, DNA storage holds immense potential to revolutionize the field with its futuristic capabilities.
Citation
● Zhang H, Deng S, Ren L, Zheng P, Hu X, Jin T, Tan X. Profiling CD8+ T cell epitopes of COVID-19 convalescents reveals reduced cellular immune responses to SARS-CoV-2 variants. Cell Rep. 2021 Sep 14;36(11).
NGSHybridization Capture DNA Probe Hybridization Capture RNA Probe QuarXeq Human All Exon Probes 3.0 HRD panel Library Preparation DNA Library Preparation Kit Fragmentation Reagent RNA Library Preparation Kit mRNA Capture Kit rRNA Depletion Kit QuarPro Superfast T4 DNA Ligase Hybridization Capture QuarHyb DNA Reagent Kit Plus Hybridization and Cleaning Solution System QuarHyb Super Reagent Kit Pro Dynegene Adapter Family Dynegene Blocker Family Multiplex PCR QuarMultiple BRCA Amplicon QuarMultiple PCR Capture Kit 2.0 PathoSeq 450 Pathogen Library Corollary Reagent Streptavidin magnetic beads Equipment and Software The iQuars50 NGS Prep System
Primers and Probes
RNA SynthesissgRNA miRNA siRNA
Gene Synthesis
Oligo Pools
CRISPR sgRNA Library
Antibody Library
Variant Library
Tel: 400-017-9077
Address: Floor 2, Building 5, No. 248 Guanghua Road, Minhang District, Shanghai
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