Highly Uniform and Accurate Synthesis

NGS sequencing verification of oligo pools with identical sequences synthesized separately by Dynegene and a competitor revealed superior uniformity in Dynegene's synthesized oligo pools.

 

Application Examples

Oligo pool synthesis is the foundation of library construction. To build a successful library, strict control is necessary throughout the synthesis process. Leveraging the high-throughput DNA synthesis platform developed by Dynegene, we constructed a library containing 63,950 sgRNA sequences. NGS verification demonstrated high coverage and uniformity of the sgRNA library.

Analysis of NGS data from four batches of oligo pool-constructed libraries revealed high coverage, uniform distribution, and excellent stability across batches of the sgRNA libraries.

 

Batch number	Diversity of designed library	Actual diversity of the library	Coverage	skew ratio
01	63950	63950	100%	2.24
02	63950	63940	99.98%	2.39
03	63950	63937	99.98%	2.91
04	63950	63943	99.99%	2.29