Ultra-multiplex PCR targeted capture is a technology that combines multiplex PCR with high-throughput sequencing, characterized by high specificity and short experimental cycle. The upgraded version of Dynegene's QuarMultiple PCR Capture Kit 2.0 exhibits superior coverage and uniformity. Moreover, the upgraded version is divided into two parts, ultra-multiplex amplification and library construction, providing more flexibility in selecting the reagent combination for experiments that require multiple pool amplification. It can be widely applied in fields such as tumor detection, pathogenic microorganism and drug resistance gene detection.