Poly(A) Tracts
As one of the complex sequence types in gene synthesis, Poly(A) tracts often presents challenges during synthesis. Dynegene's independently developed gene synthesis platform provides a good solution for synthesizing complex sequences.
Target sequence fragment
...AGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG...
Result: Both forward and reverse sequencing results are completely accurate.
Forward sequencing
Reverse sequencing
To ensure the correct quantity of PolyA, we performed bidirectional sequencing, and the results showed that both forward and reverse sequencing results were completely accurate.
Long fragment synthesis - 10KB
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTG…(447bp)…
TAATAATTTCTTGGGTAGTTTGCAGTTTTAAAATTATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTGAAAGTATTTCGATTTCTTGGCTTTATATATCTTG…(3004bp)…
AGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTGAGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGC…(8769bp)…
TTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTC
Result: The sequencing results are completely accurate.
NGSHybridization Capture DNA Probe Hybridization Capture RNA Probe QuarXeq Human All Exon Probes 3.0 HRD panel Library Preparation DNA Library Preparation Kit Fragmentation Reagent RNA Library Preparation Kit mRNA Capture Kit rRNA Depletion Kit QuarPro Superfast T4 DNA Ligase Hybridization Capture QuarHyb DNA Plus 2 Reagent Kit QuarHyb DNA Reagent Kit Plus Hybridization and Cleaning Solution System QuarHyb Super Reagent Kit Pro Dynegene Adapter Family Dynegene Blocker Family Multiplex PCR QuarMultiple BRCA Amplicon QuarMultiple PCR Capture Kit 2.0 PathoSeq 450 Pathogen Library Corollary Reagent Streptavidin magnetic beads Equipment and Software The iQuars50 NGS Prep System
Primers and Probes
RNA SynthesissgRNA miRNA siRNA
Gene Synthesis
Oligo Pools
CRISPR sgRNA Library
Antibody Library
Variant Library
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