• Blocking performance on panels of different sizes

 

Figure 1. Blocking performance of QuarAcces Universal Blocker with Cot-1 and Competing Products on Panels of Different Sizes

 

Note: Input 100 ng of standard (NA24385) was used for ultrasonic DNA library preparation. 750 ng of prepared library was used in each test, and Dynegene's blocker along with those from other manufacturers were used for routine hybridization capture (QuarHyb One Reagent Kit, Dynegene, NC1003). 10-gene (QuarXeq NCCN Cancer Panel 1.0, Dynegene, NY1016, 88kb) and WES 3.0 (QuarXeq Human All Exon Probes 1.0, Dynegene, NY1001, 41.5Mb) probes, were used for capture and sequencing on the Illumina platform. The results showed that when the capture was carried out on panels of different sizes, the blocking performance of Dynegene's blocker was slightly better than or equivalent to that of competing products.

 

• Blocking performance on different amount libraries

 

Figure 2. Blocking performance of QuarAcces Universal Blocker with Cot-1 on Different Total Amount Libraries

 

Note: A plurality of double-ended pre-index libraries were prepared. Each library was mixed with 500 ng respectively to form libraries whose total amount were 3 µg, 6 µg and 9 µg, respectively, which were subjected to overnight hybridization capture by 10-gene RNA probes (QuarXeq NCCN Cancer Panel 1.0, Dynegene, NY1016) (QuarHyb One Reagent Kit, Dynegene, NC1003), and sequencing on the Illumina platform. The results showed that Dynegene's blocker exhibited excellent and stable performance for different total amount libraries.

 

• Blocking performance of the blocker on different platforms

 

Figure 3. Comparison of Blocking performance between QuarAcces Universal Blocker with Cot-1 (Illumina platform) and Competing Products

Figure 4. Comparison of Blocking performance of QuarAcces Universal Blocker with Cot-1 for MGI Dual and Competing Products