Excellent capture performance across different panels
Using ultrasonically fragmented gDNA as the sample for library preparation (dynegene, NL1001), single-sample hybridization capture assays were performed with different panels (82kb, 540kb, 829kb, 41.5Mb). The results showed that the capture efficiency and uniformity for different panels were excellent.
Figure 1. Capture performance across different panels
Comparable performance to competing products
Using ultrasonically fragmented gDNA as the sample for library preparation (Dynegene, NL1001), hybridization capture assays were performed with a 41.5 Mb panel using Dynegene's DNA hybridization reagent NC1007 or a similar product from Company A. The sequencing results showed that Dynegene's capture reagent was comparable in performance to Company A's similar product.
Figure 2. Capture performance comparisons between Dynegene and Company A's similar product
Excellent capture performance in both single and multiple hybridizations
Using ultrasonically fragmented gDNA as the sample for library preparation (dynegene, NL1001), single and 12 samples hybridization capture assays were performed with an 829kb panel using DNA hybridization reagent NC1007. The sequencing results showed consistent capture performance in both 1- plex and 12- plex hybridizations.
Figure 3. Capture performance in single-plex and multiple-plex hybridizations
NGSHybridization Capture DNA Probe QuarStar Liquid Pan-Cancer Panel 3.0 QuarStar Pan-Cancer Lite Panel 3.0 QuarStar Pan-Cancer Fusion Panel 1.0 QuarStar Pan Cancer Panel 1.0 Hybridization Capture RNA Probe QuarXeq Human All Exon Probes 3.0 HRD panel Library Preparation DNA Library Preparation Kit Fragmentation Reagent mRNA Capture Kit rRNA Depletion Kit QuarPro Superfast T4 DNA Ligase Hybridization Capture QuarHyb Super DNA Reagent Kit QuarHyb DNA Plus 2 Reagent Kit QuarHyb DNA Reagent Kit Plus QuarHyb One Reagent Kit QuarHyb Super Reagent Kit Pro Dynegene Adapter Family Dynegene Blocker Family Multiplex PCR QuarMultiple BRCA Amplicon QuarMultiple PCR Capture Kit 2.0 PathoSeq 450 Pathogen Library Corollary Reagent Streptavidin magnetic beads Equipment and Software The iQuars50 NGS Prep System
Primers and Probes
RNA SynthesissgRNA miRNA siRNA
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CRISPR sgRNA Library
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