Dynegene Releases the World's First 350 nt ssDNA Oligonucleotide Pools

Dynegene Technologies, a leading high-throughput DNA synthesis company in China, has successfully overcome key technical challenges by integrating its independently developed high-throughput in-situ synthesis platform with optimized nucleic acid chemistry. As a result, Dynegene has officially launched the world’s first single-stranded DNA (ssDNA) oligonucleotide pools with a maximum length of 350 nucleotides (nt).

This achievement represents a major breakthrough in high-throughput oligonucleotide synthesis in terms of sequence length, uniformity, and scalability, and is expected to bring transformative advances to fields such as antibody research, aptamer discovery, AI-driven protein engineering, high-throughput gene libraries, complex sequence libraries, and synthetic biology research.

 

Dynegene’s ultra-long ssDNA oligo pool synthesis technology is capable of simultaneously producing millions of diverse, high-quality single-stranded oligonucleotides within 2–3 days. It also supports a wide range of customized designs, including variable-length sequences, arbitrary degenerate base designs, methylated bases, biotin labeling, uracil incorporation, and other specialized DNA sequence configurations.

Based on internal testing and customer feedback, the synthesized products demonstrate excellent overall quality.

 

As shown in the left figure below, polyacrylamide gel electrophoresis (PAGE) analysis of the crude synthesis products indicates high purity, uniform length distribution, and strong reproducibility of the ssDNA oligo pools.

As shown in the right figure, the same two samples were amplified and converted into double-stranded DNA (dsDNA), yielding clear and complete bands with strong amplification performance.

Next-generation sequencing (NGS) validation further confirms high sequence fidelity and a low error rate.

When these ultra-long ssDNA oligo pools were used directly as starting material to construct high-throughput single-gene libraries, Sanger sequencing verification showed a correct cloning success rate exceeding 90%.

 

 

 

Dynegene 350 nt Single-Stranded Oligo Pool

Traditional single-stranded oligo pool synthesis technologies are typically limited to sequence lengths of 100–300 nt, which restricts the achievable diversity and complexity of antibody libraries and poses significant challenges for antibody research.

As a trusted partner in high-throughput DNA synthesis, Dynegene's newly launched 350 nt ultra-long oligo pools enable the one-step synthesis of long and complex sequences. This approach eliminates the need for multi-step assembly of short oligos, reduces error accumulation, improves experimental efficiency and success rates, and offers greater design flexibility.

 

Application Scenarios Include:

 

Ultra-long Antibody Library Construction (scFv, VHH, etc.)

• Capable of covering the complete variable region of VHH antibodies or even longer regions. Complete scFv antibody sequences can be assembled by splicing just two 350 nt oligos, avoiding the complex multi-step assembly required for traditional short oligos, thereby reducing error rates and improving library diversity and screening success.

• Enables direct synthesis of full-length VHH or IgG variable domain sequences designed by AI, shortening the cycle from synthesis to functional validation and accelerating closed-loop AI-driven design.

 

High-throughput Protein Libraries and Synthetic Biology Applications

• Supports construction of high-throughput protein variant libraries covering longer coding regions (e.g., complete functional domains) for protein function optimization and engineering studies.

• Enables a broader range of long-sequence validation designs derived from AI-driven de novo design, improving the efficiency of the DBTL (Design-Build-Test-Learn) cycle in synthetic biology.

 

Low-cost High-throughput Gene Synthesis

• Uses oligo pools as starting material in combination with molecular barcoding techniques to directly construct short genes, reducing both synthesis cost and turnaround time.

• Enables high-throughput, low-cost genome assembly through oligo pool–based assembly strategies, meeting the needs of both academic research and industrial-scale applications.

 

Regulatory Elements and Functional Genomics

• Well suited for massively parallel reporter assays (MPRA), allowing direct synthesis of libraries containing longer promoters, enhancers, and regulatory elements for more comprehensive analysis of gene expression regulation networks.

• Supports synthesis and functional validation of highly repetitive regulatory regions (e.g., STRs), overcoming limitations of traditional short oligo pools that struggle with continuous repeat sequences.

 

Nucleic Acid Drug Development

• Supports construction of high-diversity aptamer libraries covering longer binding regions, improving specificity screening efficiency.

• Enables creation of mRNA optimization libraries (e.g., codon optimization, UTR modifications) for nucleic acid drug screening and development, accelerating therapeutic discovery.

 

 

Features of Dynegene 350 nt ssDNA Oligo Pools

 

• Sequence length: up to 350 nt; variable lengths supported

 

• Number of sequences: no theoretical limit

 

• Sequence composition: supports arbitrary degenerate base designs beyond the four standard nucleotides

 

• Chemical modifications: supports incorporation of 5-methylcytosine (5mC), biotin, deoxyuridine (dU), and other modifications at designated positions

 

• Turnaround time: as fast as 7 business days

 

 

The successful synthesis of 350 nt ssDNA oligo pools represents not only a technological milestone, but also opens up new experimental possibilities for researchers. Applications that were previously difficult or impractical using short oligo pools—ranging from antibody library construction and high-throughput gene assembly to regulatory sequence analysis and DNA data storage—can now be achieved directly.

 

Interested in learning more about Dynegene's 350 nt ssDNA oligo pools or customizing a solution for your application?

Contact us today!