As a leading enterprise in high-throughput DNA synthesis in China, Dynegene Technologies has successfully overcome technical barriers by integrating our proprietary high-throughput "virtual well" in-situ synthesis platform with optimized nucleic acid chemistry. We are proud to officially launch the world’s first 350nt single-stranded DNA (ssDNA) Oligo Pools.

 

This milestone represents a major breakthrough in the length, uniformity, and scalability of high-throughput oligonucleotide synthesis, bringing revolutionary transformations to fields such as antibody R&D, aptamer research, AI protein engineering, high-throughput gene libraries, complex sequence libraries, and synthetic biology.

 

Our ultra-long ssDNA Oligo Pool synthesis technology can parallel-generate millions of diverse, high-quality single-stranded oligonucleotides within 2-3 days. It supports fully customizable designs, including unequal lengths, arbitrary degenerate bases, methylated bases, biotin labeling, uracil bases, and more. Both internal testing and user feedback confirm the exceptional synthesis quality of our products.

 

As shown in the Polyacrylamide Gel Electrophoresis (PAGE) analysis of the crude product (left image), our ssDNA Oligo Pools exhibit high purity, consistent length, and high synthesis reproducibility. As shown in the right image, the conversion of two identical samples from ssDNA to dsDNA after amplification shows clear, intact bands and robust amplifiability. NGS sequencing has further verified high sequence fidelity and a low error rate. When using our ultra-long ssDNA Oligo Pools as raw materials for high-throughput direct single-gene construction, Sanger sequencing confirms a correct clone success rate of over 90%.

 

 

Dynegene 350nt ssDNA Oligo Pools

 

Traditional high-throughput synthesis of single-stranded oligonucleotide pools has often been limited by sequence length (100nt–300nt). This limitation makes it difficult to achieve ideal levels of diversity and complexity in antibody libraries, posing a significant challenge to antibody R&D.

 

As a reliable partner in the field of high-throughput DNA synthesis, Dynegene Technologies has launched the new 350nt ultra-long Oligo Pools. This technology enables the one-step synthesis of long and complex sequences, eliminating the errors and inefficiencies associated with multi-step assembly required by traditional short oligos. It significantly enhances experimental efficiency and success rates while supporting higher design flexibility. Application scenarios include:

 

Construction of ultra-long antibody libraries (scFv, VHH antibodies, etc.)

Coverage of comprehensive antibody regions: Our technology can cover the entire variable region of VHH antibodies or even longer segments, as well as the full single-domain sequences (VH or VL) of IgG antibody variable regions. Furthermore, a complete scFv sequence can be fully covered by splicing just two 350nt oligos. This bypasses the complex, multi-step assembly required by traditional short-chain oligos, thereby reducing error rates and enhancing both library diversity and screening success rates.

Direct synthesis for AI-driven design: Complete VHH sequences or single-domain sequences of IgG variable regions (VH or VL) designed by AI can be synthesized directly. This significantly shortens the cycle from sequence synthesis to functional validation, accelerating the "dry-wet loop" verification for AI-based de novo antibody design.

 

High-Throughput Protein Libraries and Synthetic Biology Applications

Construction of high-throughput protein variant libraries covering longer coding regions (e.g., complete structural domains) for protein function optimization and engineering.

Support for diverse AI-driven de novo designed long-fragment protein sequences, enhancing the efficiency of the "Design-Build-Test-Learn" (DBTL) cycle in synthetic biology.

 

Low-Cost, High-Throughput Gene Synthesis

Direct construction of short genes using oligo pools as raw materials combined with molecular barcoding technology, reducing both the cost and turnaround time for small gene fragment synthesis.

High-throughput, cost-effective gene assembly through splicing strategies using oligo pools as building blocks. This approach is tailored for the construction of large-scale gene libraries, meeting the high-throughput demands of both scientific research and industrial applications.

 

Regulatory Elements and Functional Genomics

Enhanced suitability for Massively Parallel Reporter Assay (MPRA) research, enabling the direct synthesis of sequence libraries covering longer promoters, enhancers, and regulatory elements to more comprehensively decipher gene expression regulatory networks.

Support for the synthesis and functional validation of highly repetitive regulatory regions, such as Short Tandem Repeats (STRs), overcoming the limitations of traditional short-chain oligo pools in synthesizing multiple consecutive repetitive sequences.

 

Nucleic Acid Drug Development

Support for the synthesis of high-diversity Aptamer libraries, covering longer binding region sequence designs to improve the efficiency of specificity screening for nucleic acid aptamers.

Support for the construction of mRNA optimization libraries (e.g., codon optimization, UTR region modification), tailored for functional screening scenarios and accelerating the development process of nucleic acid drugs.

 

Product Features of Dynegene 350nt ssDNA Oligo Pools

 

Sequence Length: Up to 350nt; supports variable-length sequence designs.

Number of Sequences: No limit.

Sequence Features: Supports arbitrary degenerate base designs in addition to the four standard bases.

Sequence Modifications: Supports incorporation of modifications such as 5mC, Biotin, and dU at various positions.

Turnaround Time: As fast as 7 working days.

 

The successful synthesis of 350nt ssDNA Oligo Pools is more than just a technical breakthrough; it opens up entirely new experimental possibilities for researchers. From antibody library construction to high-throughput gene assembly, and from functional studies of regulatory sequences to DNA data storage—applications that were previously beyond the reach of traditional short-chain oligo pools can now be directly realized.