Compatible with Various Hybridization Methods

QuarStar Human All Exon Probes 4.0 supports single-plex and multiplex hybridizations, with stable and excellent performance in both rapid and overnight hybridization.

Note: Library preparation was performed using Dynegene QuarPrep EZ DNA Library Kit. 500 ng of prepared library was used as input. The rapid hybridization capture experiment (0.5-2h) was conducted using QuarStar Human All Exon Probes 4.0 following the instructions of the QuarHyb Super DNA Reagent (Dynegene, Cat. # NC1009). The overnight hybridization experiment was conducted following the instructions of the Dynegene QuarHyb DNA Plus 3 Reagent Kit (Dynegene, Cat. # NC1018).

Comparison with Competitors

QuarStar Human All Exon Probes 4.0 demonstrates superior capture performance, requiring less sequencing data to achieve the same target depth.

Note: Experiments used gDNA standard (NA12878) for DNA enzymatic fragmentation library preparation (Dynegene, NL1002). 500 ng of prepared library was used as input for overnight hybridization capture experiments. Mean Depth Per Gb refers to the deduplicated mean coverage depth in the target regions per Gb of raw data. A higher value indicates that less data is required to achieve the same depth.

Variant Detection

QuarStar Human All Exon Probes 4.0 exhibits fewer false positive and false negative variants.

Table 1. NA12878 standard sample detection results.

QuarStar Human All Exon Probes 4.0 leads comparable products in Recall, Precision, and F1-Score values, achieving simultaneous improvement in both Recall and Precision, thereby reducing false positives and false negatives. For INDELs, which are more challenging to detect than SNPs, it also achieves higher Recall and Precision values. The core advantage of high Recall lies in its ability to capture the maximum number of truly existing variants. Current products often overlook the decline in Recall value when pursuing capture efficiency, leading to false negative issues that can be difficult to detect during use.

QuarStar Human All Exon Probes 4.0 can significantly reduce the missed detection rate, ensuring comprehensive mining of potential causative factors from complex genomic data, providing comprehensive and reliable molecular evidence for precision medicine, enhancing diagnostic accuracy and the scientific basis for treatment planning, and avoiding missed or misdiagnosis due to overlooked pathogenic mutations. This is particularly crucial for detecting key variants such as those in rare diseases and tumor driver mutations.