Unlocking the Potential of Custom Oligo Pools

Emerging biotechnology company Dynegene is pioneering new frontiers in synthetic biology through its innovative custom oligo pool technology. As the only enterprise in China that can commercialize ultra-high-throughput next-generation DNA synthesis, Dynegene is uniquely positioned to enable researchers across diverse fields.

The Evolution of Oligo Pools

An oligonucleotide, or oligo for short, is a short strand of synthetic DNA or RNA, typically containing 20-100 bases. Oligos serve as the basic building blocks for a wide range of molecular biology applications. They can be used individually or pooled together into custom collections of thousands of unique sequences.

Early oligo pools relied on column synthesis methods that were time-consuming, expensive, and limited in scale. The introduction of microarray and DYHOW technologies revolutionized high-throughput oligo production. However, issues with accuracy, bias, and variability challenged demanding screening applications.

Dynegene overcame these pitfalls through its proprietary DYHOW-Technology-based synthesis platform. Reaching world-class standards, Dynegene’s custom oligo pools achieve unparalleled throughput, length, accuracy, and customizability.

Design and Synthesis of Custom Oligo Pools

Dynegene’s automated platform integrates advanced molecular biology, organic chemistry, and engineering innovations to enable on-demand access to custom oligo pools on an enormous scale.

Our DYHOW technology leverages proprietary molecular self-assembly techniques. First, trillions of single-stranded oligos are synthesized in situ as parallel microdroplets on a glass substrate. Next, the oligos self-assemble into double-stranded DNA. Finally, the oligo duplexes are cleaved and eluted from the surface, yielding a complex pool containing thousands of unique sequences.

This groundbreaking approach achieves a throughput exceeding 1.5 million oligos per chip, enabling cost-efficient synthesis of highly complex custom oligo pools. Our platform also allows complete user-defined customizability of oligo sequences to empower focused or saturated mutagenesis screening libraries.

Unlocking Applications with Custom Oligo Pools

The accuracy, scalability, and flexibility of Dynegene’s custom oligo pools are unlocking breakthrough applications across synthetic biology, genomics, and molecular diagnostics:

Synthetic Biology and Metabolic Engineering

Custom oligo pools serve as the raw ingredients for writing DNA. By providing libraries containing thousands of unique gene variants, our technology accelerates the construction of synthetic gene circuits and pathways. Researchers can rapidly prototype genetic designs to enhance microbes and cell lines for industrial biomanufacturing.

CRISPR Pooled Libraries

CRISPR gene editing relies on guide RNAs to target specific locations in the genome. Pooled gRNA libraries synthesized by Dynegene enable high-throughput loss-of-function genetic screens. Identifying genes involved in disease pathways, drug resistance, and cell growth through pooled CRISPR knockout experiments.

Protein Engineering

Directed protein evolution requires screening libraries of enzyme or antibody variants. Dynegene’s oligo pools empower high-diversity mutagenesis to iteratively improve proteins. Our technology enhances molecular diagnostic assays and biotherapeutic discovery.

Molecular Diagnostics

Custom primer pools synthesized by Dynegene provide affordable targeted enrichment of hundreds of genomic regions for next-generation sequencing. Our amplicon panels lower costs and turnaround times for cancer mutation detection.

The Future of DNA Synthesis

As the pioneers bringing high-throughput in situ oligo synthesis to China, Dynegene is committed to empowering our biotechnology community. We aim to continuously improve the scalability, precision, and customizability of our platform.

If you require custom oligo pools for your synthetic biology, genomics, or molecular diagnostics workflow, let our experts guide you in designing your specialized library. Please contact us to get started. Together, we can unlock the true potential of DNA synthesis.