High-Throughput Oligo Pool Synthesis for Affordable Custom CRISPR & NGS Applications

Oligo pool synthesis has emerged as a powerful tool for high-throughput applications in synthetic biology, enabling the cost-effective generation of thousands of custom oligonucleotides in parallel. At Dynegene Technologies, we specialize in providing affordable, high-quality oligo pool synthesis services to accelerate research in areas such as CRISPR gene editing, protein engineering, and NGS target enrichment.

Established in 2018 in Shanghai, China, Dynegene Technologies has quickly become a leader in the synthetic biology industry. As the only enterprise in China capable of commercializing ultra-high-throughput next-generation DNA synthesis, we have attracted significant investments from renowned investors such as Volcanics Venture, Chaosheng Capital, MSA Capital, Boyu Capital, and ByteDance.

Affordable Custom Oligo Pools

Our state-of-the-art oligo pool synthesis platform allows us to offer highly competitive pricing without compromising on quality. By leveraging advanced semiconductor-based DNA synthesis technology, we can synthesize up to 1.5 million unique oligos on a single chip, dramatically reducing costs compared to traditional column-based synthesis methods. This makes custom oligo pools accessible for a wider range of research budgets.

At Dynegene, we understand the importance of affordability in enabling groundbreaking research. Our commitment to providing cost-effective solutions has made us a trusted partner for scientists and organizations worldwide, empowering them to push the boundaries of synthetic biology without breaking the bank.

High-Throughput Oligo Pool Synthesis Technology

Dynegene's proprietary oligo pool synthesis technology combines semiconductor manufacturing with electrochemical DNA synthesis to enable the massively parallel synthesis of custom oligo pools.

Key advantages of our oligo pool synthesis platform include:

• Synthesis of up to 1.5 million unique oligos per pool
• Oligo lengths up to 230 nucleotides
• Industry-leading uniformity and representation of oligo species
• Low error rates of less than 1 in 1000 bases

This high-throughput oligo synthesis capability forms the foundation for diverse downstream applications, enabling researchers to tackle complex biological questions with unparalleled efficiency and precision.

Oligo Pool Applications

CRISPR gRNA Libraries

One of the key applications of oligo pools is the construction of CRISPR gRNA libraries for high-throughput functional genomics screens. By designing gRNA sequences targeting every gene in the genome and synthesizing them as a pooled library, researchers can perform genome-wide CRISPR knockout, activation, or inhibition screens to identify genes involved in specific biological processes.

Dynegene offers custom gRNA library synthesis to enable cutting-edge CRISPR screens. Our expertise in oligo pool synthesis ensures the highest quality gRNA libraries, with optimal representation and minimal off-target effects. Whether you are investigating gene function, identifying drug targets, or studying disease mechanisms, our gRNA libraries provide a powerful tool to accelerate your research.

Protein Engineering

Oligo pools are a powerful tool for protein engineering applications such as directed evolution, site-saturation mutagenesis, and de novo protein design. By designing diverse libraries of gene variants and synthesizing them as an oligo pool, researchers can generate large mutant libraries for high-throughput screening and selection of optimized protein variants.

Dynegene's oligo pool synthesis services enable the creation of high-quality mutant libraries to accelerate protein engineering efforts. Our advanced synthesis technology allows for the incorporation of a wide range of codon substitutions, insertions, and deletions, providing unparalleled flexibility in library design. Whether you are evolving enzymes for industrial applications, developing novel antibodies, or designing synthetic proteins, our oligo pools provide the foundation for success.

NGS Target Enrichment

Oligo pools are increasingly being used to design custom target enrichment panels for next-generation sequencing (NGS). By synthesizing pools of capture probes targeting specific genomic regions of interest, researchers can efficiently enrich those regions prior to sequencing, reducing costs and increasing coverage depth.

We offer custom NGS target enrichment panel design and synthesis using our advanced oligo pool technology. Our experts work closely with you to design optimal capture probes, ensuring high specificity and uniform coverage across your target regions. Whether you are performing targeted resequencing, variant discovery, or epigenetic analysis, Dynegene's NGS target enrichment solutions provide a cost-effective and reliable approach to focus your sequencing efforts on the most relevant genomic regions.

Oligo Pool Quality Control

Ensuring the quality and fidelity of synthesized oligo pools is critical for successful downstream applications. Dynegene employs rigorous quality control measures throughout the oligo pool synthesis process, including:

• Proprietary synthesis chemistry optimized for high coupling efficiency and low error rates
• Automated in-line process controls to monitor synthesis quality
• Next-generation sequencing-based quality control of final oligo pool products

These QC steps allow us to consistently deliver high-quality oligo pools that meet or exceed industry standards. We take pride in our commitment to quality, ensuring that every oligo pool we produce is of the highest caliber and ready for immediate use in your experiments.

Conclusion

As a leading provider of high-throughput oligo pool synthesis services, Dynegene Technologies is committed to empowering researchers with the tools they need to push the boundaries of synthetic biology. By offering affordable, high-quality custom oligo pools for diverse applications ranging from CRISPR screens to protein engineering and NGS target enrichment, we aim to accelerate scientific discoveries and drive innovation in the field.

Our state-of-the-art technology, combined with our expertise and dedication to quality, makes Dynegene the ideal partner for your oligo pool synthesis needs. Contact us today to learn how our services can help advance your research and unlock new possibilities in synthetic biology.