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rRNA Depletion Kit

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Compatible with different inputs

 

RNA standards (Agilent, Item No.:740000-41) are sampled for the library preparation of general transcriptomics at different inputs. NL3006 (Dynegene) is used for rRNA removal. Then NL3008 (Dynegene) is used for the library preparation of general transcriptomics and the sequencing is performed on an Illumina platform. The results showed that lower residual rate of rRNA can be obtained after rRNA removal library preparation at different inputs. 

 

图2.不同投入量下rRNA去除后建库rRNA残留情况Figure 1. The residual rate of library rRNA after rRNA removal at different inputs

 

Combined with library preparation products developed by Dynegene to achieve higher library yield, lower residual rate of rRNA and higher number of genes detected

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Figure 2. Comparison of the library preparation yield after rRNA removal between Dynegene and the other supplier (A)

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Figure 3. Comparison of the residual rate of library preparation rRNA after rRNA removal between Dynegene and the other supplier (A)

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Figure 4. Comparison of Dup after rRNA removal between Dynegene and the other supplier (A)

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Figure 5. Comparison of the number of genes detected after rRNA removal between Dynegene and the other supplier (A)

 

RNA standards (Agilent, Item No.: 740000-41) are selected as samples at an input of 100ng. A complete set of RNA enrichment library preparation kits are used for the library preparation of general transcriptomics respectively according to the respective instructions of Dynegene and the other supplier (A). The results showed that Dynegene delivers higher library yield, lower residual rate of rRNA and higher number of genes detected than the other supplier (A).

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