Why do operations related to the premix of the end-repair, adaptor connection and PCR need to be performed on coolers?
When multiple samples are handled concurrently, the preparation of premixes at each step is often performed, which not only improves the speed of operations, but also eliminates the differences caused by adding reagents independently.
The main function of performing operations on coolers is to maintain the activity of the enzymes at each step and avoid a reduction in repair/ligation/amplification efficiency.
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